Close
Help




JOURNAL

Bioinformatics and Biology Insights

Gene Networks Driving Bovine Mammary Protein Synthesis During the Lactation Cycle

Submit a Paper


Bioinformatics and Biology Insights 2011:5 83-98

Original Research

Published on 04 May 2011

DOI: 10.4137/BBI.S7003


Further metadata provided in PDF



Sign up for email alerts to receive notifications of new articles published in Bioinformatics and Biology Insights

Abstract

A crucial role for both insulin and mTOR in the regulation of milk protein synthesis is emerging. Bovine mammary biopsies harvested during late-pregnancy through end of subsequent lactation were used to evaluate via quantitative PCR the expression of 44 genes involved in pathways of insulin, mTOR, AMPK, and Jak2-Stat5 signalling and also glucose and amino acid (AA) transporters. We observed an increased expression during lactation of ELF5, AA and glucose transporters, insulin signaling pathway components, MAPK14, FRAP1, EIF4EBP2, GSK3A and TSC1 among mTOR signaling-related genes. Among ribosomal components RPL22 was down-regulated. The overall data support a central role of AA and glucose transporters and insulin signaling through mTOR for the regulation of protein synthesis in bovine mammary gland. Furthermore, the existence of translational competition favoring the translation of milk protein transcripts was inferred from the combined dataset.



Downloads

PDF  (5.21 MB PDF FORMAT)

RIS citation   (ENDNOTE, REFERENCE MANAGER, PROCITE, REFWORKS)

Supplementary Files 1  (149.30 KB ZIP FORMAT)

BibTex citation   (BIBDESK, LATEX)

XML




What Your Colleagues Say About Bioinformatics and Biology Insights
The publication of our paper in Bioinformatics and Biology Insights was highly professional and very pleasant on all levels: the guidelines for authors are concise, the online submission system is user-friendly, the comments from the reviewers were insightful and improved our paper, and the preparation of the manuscript for publication was efficient. I particularly liked the fast feedback from the staff on the state of the submission and review process.
Professor Werner Braun (Department of Biochemistry and Molecular Biology, Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA)
More Testimonials

Quick Links


New article and journal news notification services